Journal: Global Spine Journal

Article Title: Needle-Knife Therapy Relieves Inflammation and Neuronal Apoptosis in Cervical Spondylotic Radiculopathy via IRE1α-XBP1 Signaling

doi: 10.1177/21925682261443920

Figure Lengend Snippet: Improvement of motor function and pain relief by needle-knife therapy in CSR rats. (A) Mechanical pain threshold (PWMT), (B) thermal pain threshold (PWTL), and (C) gait score in each group. **** P < 0.0001

Article Snippet: For pathway validation, forty rats were randomized into 5 groups (n = 8 per group): the Sham, the CSR, the CSR + NK, the CSR + IXA4 (CSR rats received intraperitoneal injection of the IRE1α activator IXA4 [HY-15845, MedChemExpress, Monmouth Junction, NJ, USA] at a dose of 10 mg/kg), and the CSR + NK + IXA4 (CSR rats received intraperitoneal injection of IXA4 followed by needle-knife therapy 20 min later) groups.

Techniques:

Journal: Global Spine Journal

Article Title: Needle-Knife Therapy Relieves Inflammation and Neuronal Apoptosis in Cervical Spondylotic Radiculopathy via IRE1α-XBP1 Signaling

doi: 10.1177/21925682261443920

Figure Lengend Snippet: Attenuation of spinal cord pathological damage and inflammatory cytokine expression by needle-knife therapy in CSR rats. (A) HE staining showing pathological changes in spinal cord tissue across groups (scale bar = 100 μm). (B, C) ELISA results of IL-6 and TNF-α levels in spinal cord tissue. (D) Immunofluorescence double staining of Iba1 and TNF-α showing microglial activation (red: Iba1; green: TNF-α; blue: DAPI; scale bar = 200 μm). **** P < 0.0001

Article Snippet: For pathway validation, forty rats were randomized into 5 groups (n = 8 per group): the Sham, the CSR, the CSR + NK, the CSR + IXA4 (CSR rats received intraperitoneal injection of the IRE1α activator IXA4 [HY-15845, MedChemExpress, Monmouth Junction, NJ, USA] at a dose of 10 mg/kg), and the CSR + NK + IXA4 (CSR rats received intraperitoneal injection of IXA4 followed by needle-knife therapy 20 min later) groups.

Techniques: Expressing, Staining, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Double Staining, Activation Assay

Journal: Global Spine Journal

Article Title: Needle-Knife Therapy Relieves Inflammation and Neuronal Apoptosis in Cervical Spondylotic Radiculopathy via IRE1α-XBP1 Signaling

doi: 10.1177/21925682261443920

Figure Lengend Snippet: Reduction of neuronal damage and inhibition of neuronal apoptosis by needle-knife therapy in CSR rats. (A) Nissl staining assessing neuronal number and morphology in spinal cord tissue (scale bar = 200 μm). (B/C) TUNEL and NeuN dual immunofluorescence staining showing neuronal apoptosis (red: TUNEL-positive cells; green: NeuN-positive cells; blue: DAPI), scale bar = 200 μm. (D-F) qRT-PCR (D/E) and WB (F) results of Bax and Bcl-2 expression at mRNA and protein levels. **** P < 0.0001

Article Snippet: For pathway validation, forty rats were randomized into 5 groups (n = 8 per group): the Sham, the CSR, the CSR + NK, the CSR + IXA4 (CSR rats received intraperitoneal injection of the IRE1α activator IXA4 [HY-15845, MedChemExpress, Monmouth Junction, NJ, USA] at a dose of 10 mg/kg), and the CSR + NK + IXA4 (CSR rats received intraperitoneal injection of IXA4 followed by needle-knife therapy 20 min later) groups.

Techniques: Inhibition, Staining, TUNEL Assay, Immunofluorescence, Quantitative RT-PCR, Expressing

Journal: Global Spine Journal

Article Title: Needle-Knife Therapy Relieves Inflammation and Neuronal Apoptosis in Cervical Spondylotic Radiculopathy via IRE1α-XBP1 Signaling

doi: 10.1177/21925682261443920

Figure Lengend Snippet: Downregulation of key proteins in the IRE1α-XBP1 signaling in the spinal cord of CSR rats by needle-knife therapy. WB analysis of p-IRE1α and XBP1s protein levels across groups. **** P < 0.0001

Article Snippet: For pathway validation, forty rats were randomized into 5 groups (n = 8 per group): the Sham, the CSR, the CSR + NK, the CSR + IXA4 (CSR rats received intraperitoneal injection of the IRE1α activator IXA4 [HY-15845, MedChemExpress, Monmouth Junction, NJ, USA] at a dose of 10 mg/kg), and the CSR + NK + IXA4 (CSR rats received intraperitoneal injection of IXA4 followed by needle-knife therapy 20 min later) groups.

Techniques:

Journal: Global Spine Journal

Article Title: Needle-Knife Therapy Relieves Inflammation and Neuronal Apoptosis in Cervical Spondylotic Radiculopathy via IRE1α-XBP1 Signaling

doi: 10.1177/21925682261443920

Figure Lengend Snippet: Alleviation of motor dysfunction and pain through inhibition of the IRE1α-XBP1 signaling pathway by needle-knife therapy in CSR rats. (A) WB verification of the effect of IXA4 on p-IRE1α and XBP1-s levels in spinal cord tissue. (B–D) Mechanical pain threshold (PWMT), (C) thermal pain threshold (PWTL), and (D) gait score following IXA4 administration in CSR rats with or without needle-knife therapy. **** P < 0.0001

Article Snippet: For pathway validation, forty rats were randomized into 5 groups (n = 8 per group): the Sham, the CSR, the CSR + NK, the CSR + IXA4 (CSR rats received intraperitoneal injection of the IRE1α activator IXA4 [HY-15845, MedChemExpress, Monmouth Junction, NJ, USA] at a dose of 10 mg/kg), and the CSR + NK + IXA4 (CSR rats received intraperitoneal injection of IXA4 followed by needle-knife therapy 20 min later) groups.

Techniques: Inhibition

Journal: Global Spine Journal

Article Title: Needle-Knife Therapy Relieves Inflammation and Neuronal Apoptosis in Cervical Spondylotic Radiculopathy via IRE1α-XBP1 Signaling

doi: 10.1177/21925682261443920

Figure Lengend Snippet: Inhibition of pathological damage, inflammation by needle-knife therapy via suppression of the IRE1α-XBP1 signaling. (A) HE staining evaluating pathological changes in spinal cord tissue after IXA4 treatment (scale bar = 200 μm). (B, C) ELISA analysis of IL-6 and TNF-α levels following IXA4 intervention. (D) Immunofluorescence detection of microglial activation following IXA4 treatment. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: For pathway validation, forty rats were randomized into 5 groups (n = 8 per group): the Sham, the CSR, the CSR + NK, the CSR + IXA4 (CSR rats received intraperitoneal injection of the IRE1α activator IXA4 [HY-15845, MedChemExpress, Monmouth Junction, NJ, USA] at a dose of 10 mg/kg), and the CSR + NK + IXA4 (CSR rats received intraperitoneal injection of IXA4 followed by needle-knife therapy 20 min later) groups.

Techniques: Inhibition, Staining, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Activation Assay

Journal: Global Spine Journal

Article Title: Needle-Knife Therapy Relieves Inflammation and Neuronal Apoptosis in Cervical Spondylotic Radiculopathy via IRE1α-XBP1 Signaling

doi: 10.1177/21925682261443920

Figure Lengend Snippet: Inhibition of neuronal apoptosis by needle-knife therapy via suppression of the IRE1α-XBP1 signaling. (A/B) Nissl staining of spinal cord neurons after needle-knife therapy with or without IXA4, scale bar = 200 μm. (C/D) TUNEL/NeuN dual immunofluorescence staining assessing neuronal apoptosis after IXA4 treatment, scale bar = 200 μm (red: TUNEL-positive cells; green: NeuN-positive cells; blue: DAPI). (E/F) qRT-PCR and WB detection of Bax and Bcl-2 expression in spinal cord tissue. * P < 0.05, ** P < 0.01, **** P < 0.0001

Article Snippet: For pathway validation, forty rats were randomized into 5 groups (n = 8 per group): the Sham, the CSR, the CSR + NK, the CSR + IXA4 (CSR rats received intraperitoneal injection of the IRE1α activator IXA4 [HY-15845, MedChemExpress, Monmouth Junction, NJ, USA] at a dose of 10 mg/kg), and the CSR + NK + IXA4 (CSR rats received intraperitoneal injection of IXA4 followed by needle-knife therapy 20 min later) groups.

Techniques: Inhibition, Staining, TUNEL Assay, Immunofluorescence, Quantitative RT-PCR, Expressing